lambda protein phosphatase 400 units Search Results


λ phosphatase  (New England Biolabs)
96
New England Biolabs λ phosphatase
λ Phosphatase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/λ phosphatase/product/New England Biolabs
Average 96 stars, based on 1 article reviews
λ phosphatase - by Bioz Stars, 2026-03
96/100 stars
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lambda phosphatase  (New England Biolabs)
96
New England Biolabs lambda phosphatase
Lambda Phosphatase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lambda phosphatase/product/New England Biolabs
Average 96 stars, based on 1 article reviews
lambda phosphatase - by Bioz Stars, 2026-03
96/100 stars
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lambda protein phosphatase  (Millipore)
90
Millipore lambda protein phosphatase
(A) Immunoprecipitation of pAkt and pERK1/2 in lysates of control and hydrogen peroxide-treated wt and #1 cells. Immunoprecipitation was performed with anti-pAkt, anti-pERK1/2 and control IgG antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. (B) The effect of Akt inhibitor VIII (left panel) and MEK1/2 inhibitor (UO126) (right panel) on the level of poly(ADP-ribosyl)ation in control wt and #1 cells using immunoblot analysis with anti-PAR antibody (lower panel). The inhibition of kinases was estimated by immunoblot analysis with anti-pAkt, anti-pERK1/2, anti-Akt and anti-ERK1/2 antibodies (top panel). (C) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells and lysates that were dephosphorylated <t>by</t> <t>lambda-phosphatase.</t> (D) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells, without pretreatment or pretreated with Akt inhibitor VIII or MEK1/2 inhibitor (UO126). Every bar graph is accompanied by a corresponding immunoprecipitate; Immunoprecipitation was performed with anti-pAkt and/or anti-pERK1/2 antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. The results are expressed as means ± S.E.M. Means not sharing a common letter are significantly different between groups (p<0.05).
Lambda Protein Phosphatase, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lambda protein phosphatase/product/Millipore
Average 90 stars, based on 1 article reviews
lambda protein phosphatase - by Bioz Stars, 2026-03
90/100 stars
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lambda protein phosphatase 400 units  (Millipore)
90
Millipore lambda protein phosphatase 400 units
(A) Immunoprecipitation of pAkt and pERK1/2 in lysates of control and hydrogen peroxide-treated wt and #1 cells. Immunoprecipitation was performed with anti-pAkt, anti-pERK1/2 and control IgG antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. (B) The effect of Akt inhibitor VIII (left panel) and MEK1/2 inhibitor (UO126) (right panel) on the level of poly(ADP-ribosyl)ation in control wt and #1 cells using immunoblot analysis with anti-PAR antibody (lower panel). The inhibition of kinases was estimated by immunoblot analysis with anti-pAkt, anti-pERK1/2, anti-Akt and anti-ERK1/2 antibodies (top panel). (C) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells and lysates that were dephosphorylated <t>by</t> <t>lambda-phosphatase.</t> (D) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells, without pretreatment or pretreated with Akt inhibitor VIII or MEK1/2 inhibitor (UO126). Every bar graph is accompanied by a corresponding immunoprecipitate; Immunoprecipitation was performed with anti-pAkt and/or anti-pERK1/2 antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. The results are expressed as means ± S.E.M. Means not sharing a common letter are significantly different between groups (p<0.05).
Lambda Protein Phosphatase 400 Units, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lambda protein phosphatase 400 units/product/Millipore
Average 90 stars, based on 1 article reviews
lambda protein phosphatase 400 units - by Bioz Stars, 2026-03
90/100 stars
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recombinant lambda protein phosphatase 14–405  (Millipore)
90
Millipore recombinant lambda protein phosphatase 14–405
(A) Immunoprecipitation of pAkt and pERK1/2 in lysates of control and hydrogen peroxide-treated wt and #1 cells. Immunoprecipitation was performed with anti-pAkt, anti-pERK1/2 and control IgG antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. (B) The effect of Akt inhibitor VIII (left panel) and MEK1/2 inhibitor (UO126) (right panel) on the level of poly(ADP-ribosyl)ation in control wt and #1 cells using immunoblot analysis with anti-PAR antibody (lower panel). The inhibition of kinases was estimated by immunoblot analysis with anti-pAkt, anti-pERK1/2, anti-Akt and anti-ERK1/2 antibodies (top panel). (C) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells and lysates that were dephosphorylated <t>by</t> <t>lambda-phosphatase.</t> (D) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells, without pretreatment or pretreated with Akt inhibitor VIII or MEK1/2 inhibitor (UO126). Every bar graph is accompanied by a corresponding immunoprecipitate; Immunoprecipitation was performed with anti-pAkt and/or anti-pERK1/2 antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. The results are expressed as means ± S.E.M. Means not sharing a common letter are significantly different between groups (p<0.05).
Recombinant Lambda Protein Phosphatase 14–405, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant lambda protein phosphatase 14–405/product/Millipore
Average 90 stars, based on 1 article reviews
recombinant lambda protein phosphatase 14–405 - by Bioz Stars, 2026-03
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lambda protein phosphatase (ppase  (Millipore)
90
Millipore lambda protein phosphatase (ppase
(A) Immunoprecipitation of pAkt and pERK1/2 in lysates of control and hydrogen peroxide-treated wt and #1 cells. Immunoprecipitation was performed with anti-pAkt, anti-pERK1/2 and control IgG antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. (B) The effect of Akt inhibitor VIII (left panel) and MEK1/2 inhibitor (UO126) (right panel) on the level of poly(ADP-ribosyl)ation in control wt and #1 cells using immunoblot analysis with anti-PAR antibody (lower panel). The inhibition of kinases was estimated by immunoblot analysis with anti-pAkt, anti-pERK1/2, anti-Akt and anti-ERK1/2 antibodies (top panel). (C) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells and lysates that were dephosphorylated <t>by</t> <t>lambda-phosphatase.</t> (D) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells, without pretreatment or pretreated with Akt inhibitor VIII or MEK1/2 inhibitor (UO126). Every bar graph is accompanied by a corresponding immunoprecipitate; Immunoprecipitation was performed with anti-pAkt and/or anti-pERK1/2 antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. The results are expressed as means ± S.E.M. Means not sharing a common letter are significantly different between groups (p<0.05).
Lambda Protein Phosphatase (Ppase, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lambda protein phosphatase (ppase/product/Millipore
Average 90 stars, based on 1 article reviews
lambda protein phosphatase (ppase - by Bioz Stars, 2026-03
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λ phosphatase  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology λ phosphatase
(A) Immunoprecipitation of pAkt and pERK1/2 in lysates of control and hydrogen peroxide-treated wt and #1 cells. Immunoprecipitation was performed with anti-pAkt, anti-pERK1/2 and control IgG antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. (B) The effect of Akt inhibitor VIII (left panel) and MEK1/2 inhibitor (UO126) (right panel) on the level of poly(ADP-ribosyl)ation in control wt and #1 cells using immunoblot analysis with anti-PAR antibody (lower panel). The inhibition of kinases was estimated by immunoblot analysis with anti-pAkt, anti-pERK1/2, anti-Akt and anti-ERK1/2 antibodies (top panel). (C) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells and lysates that were dephosphorylated <t>by</t> <t>lambda-phosphatase.</t> (D) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells, without pretreatment or pretreated with Akt inhibitor VIII or MEK1/2 inhibitor (UO126). Every bar graph is accompanied by a corresponding immunoprecipitate; Immunoprecipitation was performed with anti-pAkt and/or anti-pERK1/2 antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. The results are expressed as means ± S.E.M. Means not sharing a common letter are significantly different between groups (p<0.05).
λ Phosphatase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/λ phosphatase/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
λ phosphatase - by Bioz Stars, 2026-03
93/100 stars
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lambda protein phosphatase 200–400 units  (Millipore)
90
Millipore lambda protein phosphatase 200–400 units
(A) Immunoprecipitation of pAkt and pERK1/2 in lysates of control and hydrogen peroxide-treated wt and #1 cells. Immunoprecipitation was performed with anti-pAkt, anti-pERK1/2 and control IgG antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. (B) The effect of Akt inhibitor VIII (left panel) and MEK1/2 inhibitor (UO126) (right panel) on the level of poly(ADP-ribosyl)ation in control wt and #1 cells using immunoblot analysis with anti-PAR antibody (lower panel). The inhibition of kinases was estimated by immunoblot analysis with anti-pAkt, anti-pERK1/2, anti-Akt and anti-ERK1/2 antibodies (top panel). (C) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells and lysates that were dephosphorylated <t>by</t> <t>lambda-phosphatase.</t> (D) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells, without pretreatment or pretreated with Akt inhibitor VIII or MEK1/2 inhibitor (UO126). Every bar graph is accompanied by a corresponding immunoprecipitate; Immunoprecipitation was performed with anti-pAkt and/or anti-pERK1/2 antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. The results are expressed as means ± S.E.M. Means not sharing a common letter are significantly different between groups (p<0.05).
Lambda Protein Phosphatase 200–400 Units, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lambda protein phosphatase 200–400 units/product/Millipore
Average 90 stars, based on 1 article reviews
lambda protein phosphatase 200–400 units - by Bioz Stars, 2026-03
90/100 stars
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nmr spectrometer jeol lambda 400  (JEOL)
90
JEOL nmr spectrometer jeol lambda 400
(A) Immunoprecipitation of pAkt and pERK1/2 in lysates of control and hydrogen peroxide-treated wt and #1 cells. Immunoprecipitation was performed with anti-pAkt, anti-pERK1/2 and control IgG antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. (B) The effect of Akt inhibitor VIII (left panel) and MEK1/2 inhibitor (UO126) (right panel) on the level of poly(ADP-ribosyl)ation in control wt and #1 cells using immunoblot analysis with anti-PAR antibody (lower panel). The inhibition of kinases was estimated by immunoblot analysis with anti-pAkt, anti-pERK1/2, anti-Akt and anti-ERK1/2 antibodies (top panel). (C) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells and lysates that were dephosphorylated <t>by</t> <t>lambda-phosphatase.</t> (D) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells, without pretreatment or pretreated with Akt inhibitor VIII or MEK1/2 inhibitor (UO126). Every bar graph is accompanied by a corresponding immunoprecipitate; Immunoprecipitation was performed with anti-pAkt and/or anti-pERK1/2 antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. The results are expressed as means ± S.E.M. Means not sharing a common letter are significantly different between groups (p<0.05).
Nmr Spectrometer Jeol Lambda 400, supplied by JEOL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nmr spectrometer jeol lambda 400/product/JEOL
Average 90 stars, based on 1 article reviews
nmr spectrometer jeol lambda 400 - by Bioz Stars, 2026-03
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il 28a ifn lambda 2 antibody mm0400 6z37 dylight 755  (Novus Biologicals)
N/A
The IL 28A IFN lambda 2 Antibody MM0400 6Z37 DyLight 755 from Novus Biologicals is a mouse monoclonal antibody to IL 28A IFN lambda 2 This antibody reacts with human The IL 28A IFN lambda
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il 28a ifn lambda 2 antibody mm0400 6z37 dylight 680  (Novus Biologicals)
N/A
The IL 28A IFN lambda 2 Antibody MM0400 6Z37 DyLight 680 from Novus Biologicals is a mouse monoclonal antibody to IL 28A IFN lambda 2 This antibody reacts with human The IL 28A IFN lambda
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il 28a ifn lambda 2 antibody mm0400 6z37 alexa fluor« 700  (Novus Biologicals)
N/A
The IL 28A IFN lambda 2 Antibody MM0400 6Z37 Alexa Fluor« 700 from Novus Biologicals is a mouse monoclonal antibody to IL 28A IFN lambda 2 This antibody reacts with human The IL 28A IFN
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Image Search Results


(A) Immunoprecipitation of pAkt and pERK1/2 in lysates of control and hydrogen peroxide-treated wt and #1 cells. Immunoprecipitation was performed with anti-pAkt, anti-pERK1/2 and control IgG antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. (B) The effect of Akt inhibitor VIII (left panel) and MEK1/2 inhibitor (UO126) (right panel) on the level of poly(ADP-ribosyl)ation in control wt and #1 cells using immunoblot analysis with anti-PAR antibody (lower panel). The inhibition of kinases was estimated by immunoblot analysis with anti-pAkt, anti-pERK1/2, anti-Akt and anti-ERK1/2 antibodies (top panel). (C) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells and lysates that were dephosphorylated by lambda-phosphatase. (D) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells, without pretreatment or pretreated with Akt inhibitor VIII or MEK1/2 inhibitor (UO126). Every bar graph is accompanied by a corresponding immunoprecipitate; Immunoprecipitation was performed with anti-pAkt and/or anti-pERK1/2 antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. The results are expressed as means ± S.E.M. Means not sharing a common letter are significantly different between groups (p<0.05).

Journal: PLoS ONE

Article Title: CXC Chemokine Ligand 12 Protects Pancreatic β-Cells from Necrosis through Akt Kinase-Mediated Modulation of Poly(ADP-ribose) Polymerase-1 Activity

doi: 10.1371/journal.pone.0101172

Figure Lengend Snippet: (A) Immunoprecipitation of pAkt and pERK1/2 in lysates of control and hydrogen peroxide-treated wt and #1 cells. Immunoprecipitation was performed with anti-pAkt, anti-pERK1/2 and control IgG antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. (B) The effect of Akt inhibitor VIII (left panel) and MEK1/2 inhibitor (UO126) (right panel) on the level of poly(ADP-ribosyl)ation in control wt and #1 cells using immunoblot analysis with anti-PAR antibody (lower panel). The inhibition of kinases was estimated by immunoblot analysis with anti-pAkt, anti-pERK1/2, anti-Akt and anti-ERK1/2 antibodies (top panel). (C) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells and lysates that were dephosphorylated by lambda-phosphatase. (D) PARP-1 activity assay performed with nuclear lysates from control and hydrogen peroxide-treated #1 cells, without pretreatment or pretreated with Akt inhibitor VIII or MEK1/2 inhibitor (UO126). Every bar graph is accompanied by a corresponding immunoprecipitate; Immunoprecipitation was performed with anti-pAkt and/or anti-pERK1/2 antibodies, and the obtained immunoprecipitates were probed with anti-PARP-1 antibody. The results are expressed as means ± S.E.M. Means not sharing a common letter are significantly different between groups (p<0.05).

Article Snippet: Dephosphorylation was performed with 100 µg of Rin-5F nuclear lysates that were incubated for 20 min at 30°C with 400 units of lambda protein phosphatase (Sigma, Saint Louis, MO, USA) in reaction buffer supplemented with 1× MnCl 2 , all provided by the manufacturer.

Techniques: Immunoprecipitation, Western Blot, Inhibition, Activity Assay