lambda protein phosphatase 400 units Search Results


lambda λ phosphatase  (New England Biolabs)
97
New England Biolabs lambda λ phosphatase
( A ) Western blot of IncV-3xFLAG from lysates of HEK293 cells expressing IncV-3xFLAG (293), HEK293 cells infected with C. trachomatis expressing IncV-3xFLAG (293+ Ct ), or E. coli expressing IncV-3xFLAG ( Ec ). ( B ) Western blot of IncV-3xFLAG purified from lysates of HEK293 cells infected with C. trachomatis expressing IncV-3xFLAG and treated with <t>lambda</t> (λ) phosphatase (+) or phosphatase buffer alone (−). ( C ) Three-dimensional reconstruction of confocal images of HeLa cells infected with C. trachomatis expressing mCherry constitutively (red) and IncV-3xFLAG (blue) under the control of an anhydrotetracycline (aTc)-inducible promoter in the absence (−aTc) or presence (+aTc) of aTc and stained to detect endogenous CK2β (yellow). The merge is shown on the right. Scale bar is 5 μm. ( D ) In vitro kinase assay using GST or GST-IncV 167-363 purified from E. coli as a substrate in the presence (+) or absence (−) of recombinant CK2 and in the presence (+) or absence (−) of ATPγS. The top panel shows phosphorylated proteins detected with anti-thiophosphate antibodies and the bottom panel is the same membrane stained with Ponceau S to detect total proteins. Figure 1—source data 1. Uncropped, labeled blots for . Figure 1—source data 2. Uncropped, labeled blots for . Figure 1—source data 3. Uncropped, labelled blots for . Figure 1—source data 4. Raw data for FLAG blot in . Figure 1—source data 5. Raw data for FLAG blot in . Figure 1—source data 6. Raw data for thiophosphate blot 1 in . Figure 1—source data 7. Raw data for thiophosphate blot 2 in . Figure 1—source data 8. Raw data for Ponceau S blot 1 in . Figure 1—source data 9. Raw data for Ponceau S blot 2 in .
Lambda λ Phosphatase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+protein+phosphatase+400+units/pmc09286742-293-26-29?v=New+England+Biolabs
Average 97 stars, based on 1 article reviews
lambda λ phosphatase - by Bioz Stars, 2026-07
97/100 stars
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lambda phosphatase  (New England Biolabs)
97
New England Biolabs lambda phosphatase
( A ) Western blot of IncV-3xFLAG from lysates of HEK293 cells expressing IncV-3xFLAG (293), HEK293 cells infected with C. trachomatis expressing IncV-3xFLAG (293+ Ct ), or E. coli expressing IncV-3xFLAG ( Ec ). ( B ) Western blot of IncV-3xFLAG purified from lysates of HEK293 cells infected with C. trachomatis expressing IncV-3xFLAG and treated with <t>lambda</t> (λ) phosphatase (+) or phosphatase buffer alone (−). ( C ) Three-dimensional reconstruction of confocal images of HeLa cells infected with C. trachomatis expressing mCherry constitutively (red) and IncV-3xFLAG (blue) under the control of an anhydrotetracycline (aTc)-inducible promoter in the absence (−aTc) or presence (+aTc) of aTc and stained to detect endogenous CK2β (yellow). The merge is shown on the right. Scale bar is 5 μm. ( D ) In vitro kinase assay using GST or GST-IncV 167-363 purified from E. coli as a substrate in the presence (+) or absence (−) of recombinant CK2 and in the presence (+) or absence (−) of ATPγS. The top panel shows phosphorylated proteins detected with anti-thiophosphate antibodies and the bottom panel is the same membrane stained with Ponceau S to detect total proteins. Figure 1—source data 1. Uncropped, labeled blots for . Figure 1—source data 2. Uncropped, labeled blots for . Figure 1—source data 3. Uncropped, labelled blots for . Figure 1—source data 4. Raw data for FLAG blot in . Figure 1—source data 5. Raw data for FLAG blot in . Figure 1—source data 6. Raw data for thiophosphate blot 1 in . Figure 1—source data 7. Raw data for thiophosphate blot 2 in . Figure 1—source data 8. Raw data for Ponceau S blot 1 in . Figure 1—source data 9. Raw data for Ponceau S blot 2 in .
Lambda Phosphatase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+protein+phosphatase+400+units/10__1074_slash_jbc__m704554200-72-49-51?v=New+England+Biolabs
Average 97 stars, based on 1 article reviews
lambda phosphatase - by Bioz Stars, 2026-07
97/100 stars
  Buy from Supplier
λ phosphatase  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology λ phosphatase
( A ) Western blot of IncV-3xFLAG from lysates of HEK293 cells expressing IncV-3xFLAG (293), HEK293 cells infected with C. trachomatis expressing IncV-3xFLAG (293+ Ct ), or E. coli expressing IncV-3xFLAG ( Ec ). ( B ) Western blot of IncV-3xFLAG purified from lysates of HEK293 cells infected with C. trachomatis expressing IncV-3xFLAG and treated with <t>lambda</t> (λ) phosphatase (+) or phosphatase buffer alone (−). ( C ) Three-dimensional reconstruction of confocal images of HeLa cells infected with C. trachomatis expressing mCherry constitutively (red) and IncV-3xFLAG (blue) under the control of an anhydrotetracycline (aTc)-inducible promoter in the absence (−aTc) or presence (+aTc) of aTc and stained to detect endogenous CK2β (yellow). The merge is shown on the right. Scale bar is 5 μm. ( D ) In vitro kinase assay using GST or GST-IncV 167-363 purified from E. coli as a substrate in the presence (+) or absence (−) of recombinant CK2 and in the presence (+) or absence (−) of ATPγS. The top panel shows phosphorylated proteins detected with anti-thiophosphate antibodies and the bottom panel is the same membrane stained with Ponceau S to detect total proteins. Figure 1—source data 1. Uncropped, labeled blots for . Figure 1—source data 2. Uncropped, labeled blots for . Figure 1—source data 3. Uncropped, labelled blots for . Figure 1—source data 4. Raw data for FLAG blot in . Figure 1—source data 5. Raw data for FLAG blot in . Figure 1—source data 6. Raw data for thiophosphate blot 1 in . Figure 1—source data 7. Raw data for thiophosphate blot 2 in . Figure 1—source data 8. Raw data for Ponceau S blot 1 in . Figure 1—source data 9. Raw data for Ponceau S blot 2 in .
λ Phosphatase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lambda+protein+phosphatase+400+units/pmc05581041-139-24-42?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
λ phosphatase - by Bioz Stars, 2026-07
93/100 stars
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il 28a ifn lambda 2 antibody mm0400 6z37 dylight 755  (Novus Biologicals)
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The IL 28A IFN lambda 2 Antibody MM0400 6Z37 DyLight 755 from Novus Biologicals is a mouse monoclonal antibody to IL 28A IFN lambda 2 This antibody reacts with human The IL 28A IFN lambda
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il 28a ifn lambda 2 antibody mm0400 6z37 dylight 680  (Novus Biologicals)
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The IL 28A IFN lambda 2 Antibody MM0400 6Z37 DyLight 680 from Novus Biologicals is a mouse monoclonal antibody to IL 28A IFN lambda 2 This antibody reacts with human The IL 28A IFN lambda
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il 28a ifn lambda 2 antibody mm0400 6z37 alexa fluor« 700  (Novus Biologicals)
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The IL 28A IFN lambda 2 Antibody MM0400 6Z37 Alexa Fluor« 700 from Novus Biologicals is a mouse monoclonal antibody to IL 28A IFN lambda 2 This antibody reacts with human The IL 28A IFN
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il 28a ifn lambda 2 antibody mm0400 6z37  (Novus Biologicals)
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The IL 28A IFN lambda 2 Antibody MM0400 6Z37 from Novus Biologicals is a mouse monoclonal antibody to IL 28A IFN lambda 2 This antibody reacts with human The IL 28A IFN lambda 2 Antibody
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il 28a ifn lambda 2 antibody mm0400 6z37 biotin  (Novus Biologicals)
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The IL 28A IFN lambda 2 Antibody MM0400 6Z37 Biotin from Novus Biologicals is a mouse monoclonal antibody to IL 28A IFN lambda 2 This antibody reacts with human The IL 28A IFN lambda 2
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il 28a ifn lambda 2 antibody mm0400 6z37 dylight 550  (Novus Biologicals)
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The IL 28A IFN lambda 2 Antibody MM0400 6Z37 DyLight 550 from Novus Biologicals is a mouse monoclonal antibody to IL 28A IFN lambda 2 This antibody reacts with human The IL 28A IFN lambda
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phospholamban pln nm 002667 human over expression lysate  (OriGene)
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PLN HEK293T cell transient overexpression lysate as WB positive control
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il 28a ifn lambda 2 antibody mm0400 6z37 dylight 405  (Novus Biologicals)
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The IL 28A IFN lambda 2 Antibody MM0400 6Z37 DyLight 405 from Novus Biologicals is a mouse monoclonal antibody to IL 28A IFN lambda 2 This antibody reacts with human The IL 28A IFN lambda
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il 28a ifn lambda 2 antibody mm0400 6z37 alexa fluor« 488  (Novus Biologicals)
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The IL 28A IFN lambda 2 Antibody MM0400 6Z37 Alexa Fluor« 488 from Novus Biologicals is a mouse monoclonal antibody to IL 28A IFN lambda 2 This antibody reacts with human The IL 28A IFN
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Image Search Results


( A ) Western blot of IncV-3xFLAG from lysates of HEK293 cells expressing IncV-3xFLAG (293), HEK293 cells infected with C. trachomatis expressing IncV-3xFLAG (293+ Ct ), or E. coli expressing IncV-3xFLAG ( Ec ). ( B ) Western blot of IncV-3xFLAG purified from lysates of HEK293 cells infected with C. trachomatis expressing IncV-3xFLAG and treated with lambda (λ) phosphatase (+) or phosphatase buffer alone (−). ( C ) Three-dimensional reconstruction of confocal images of HeLa cells infected with C. trachomatis expressing mCherry constitutively (red) and IncV-3xFLAG (blue) under the control of an anhydrotetracycline (aTc)-inducible promoter in the absence (−aTc) or presence (+aTc) of aTc and stained to detect endogenous CK2β (yellow). The merge is shown on the right. Scale bar is 5 μm. ( D ) In vitro kinase assay using GST or GST-IncV 167-363 purified from E. coli as a substrate in the presence (+) or absence (−) of recombinant CK2 and in the presence (+) or absence (−) of ATPγS. The top panel shows phosphorylated proteins detected with anti-thiophosphate antibodies and the bottom panel is the same membrane stained with Ponceau S to detect total proteins. Figure 1—source data 1. Uncropped, labeled blots for . Figure 1—source data 2. Uncropped, labeled blots for . Figure 1—source data 3. Uncropped, labelled blots for . Figure 1—source data 4. Raw data for FLAG blot in . Figure 1—source data 5. Raw data for FLAG blot in . Figure 1—source data 6. Raw data for thiophosphate blot 1 in . Figure 1—source data 7. Raw data for thiophosphate blot 2 in . Figure 1—source data 8. Raw data for Ponceau S blot 1 in . Figure 1—source data 9. Raw data for Ponceau S blot 2 in .

Journal: eLife

Article Title: Phosphoregulation accommodates Type III secretion and assembly of a tether of ER- Chlamydia inclusion membrane contact sites

doi: 10.7554/eLife.74535

Figure Lengend Snippet: ( A ) Western blot of IncV-3xFLAG from lysates of HEK293 cells expressing IncV-3xFLAG (293), HEK293 cells infected with C. trachomatis expressing IncV-3xFLAG (293+ Ct ), or E. coli expressing IncV-3xFLAG ( Ec ). ( B ) Western blot of IncV-3xFLAG purified from lysates of HEK293 cells infected with C. trachomatis expressing IncV-3xFLAG and treated with lambda (λ) phosphatase (+) or phosphatase buffer alone (−). ( C ) Three-dimensional reconstruction of confocal images of HeLa cells infected with C. trachomatis expressing mCherry constitutively (red) and IncV-3xFLAG (blue) under the control of an anhydrotetracycline (aTc)-inducible promoter in the absence (−aTc) or presence (+aTc) of aTc and stained to detect endogenous CK2β (yellow). The merge is shown on the right. Scale bar is 5 μm. ( D ) In vitro kinase assay using GST or GST-IncV 167-363 purified from E. coli as a substrate in the presence (+) or absence (−) of recombinant CK2 and in the presence (+) or absence (−) of ATPγS. The top panel shows phosphorylated proteins detected with anti-thiophosphate antibodies and the bottom panel is the same membrane stained with Ponceau S to detect total proteins. Figure 1—source data 1. Uncropped, labeled blots for . Figure 1—source data 2. Uncropped, labeled blots for . Figure 1—source data 3. Uncropped, labelled blots for . Figure 1—source data 4. Raw data for FLAG blot in . Figure 1—source data 5. Raw data for FLAG blot in . Figure 1—source data 6. Raw data for thiophosphate blot 1 in . Figure 1—source data 7. Raw data for thiophosphate blot 2 in . Figure 1—source data 8. Raw data for Ponceau S blot 1 in . Figure 1—source data 9. Raw data for Ponceau S blot 2 in .

Article Snippet: 20 μl of eluate was combined with 2.5 μl of 10 mM MnCl 2 , 2.5 μl of 10× PMP buffer (NEB), and 400 units of lambda (λ) phosphatase (NEB) for 24 hr at 4°C.

Techniques: Western Blot, Expressing, Infection, Purification, Staining, In Vitro, Kinase Assay, Recombinant, Labeling